Scientist

Compensation

: $92,985.00 - $157,310.00 /year *

Employment Type

: Full-Time

Industry

: Scientific Research



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ROLE SUMMARY

The incumbent contributes to programs that apply molecular biology and genetics to the modification of microorganisms and cell lines that express vaccine antigens of interest. Studies directed toward improving the growth and productivity of bacterial antigen production strains is a major responsibility of the position. Support of a program that seeks to optimize in vitro transcription and template preparation is also in scope. The position is also responsible for preparing plasmids for expression of recombinant proteins, primarily in E coli. Responsibilities also include the systematic cell banking and archiving of bacterial strains. Experience in microbiology and genetics is required. An appreciation of cell banking strategies would be helpful. Experience in RNA extraction/preparation and in vitro transcription to support the design of processes for bulk production of RNA DS would be highly desirable as well. The position will pivot to fermentation and cell culture to analyze strains and as such a background in either would be extremely desirable.

ROLE RESPONSIBILITIES

  • Performs experiments designed to optimize the preparation of DNA templates and in vitro transcription reactions for the production of DS RNAs
  • The incumbent will participate in the design of recombinant plasmids for strain construction and protein expression
  • The incumbent will be responsible for the preparation of bulk plasmid preparations for use in mammalian transfection experiments and in vitro transcription
  • Prepare microbial cell banks and proactively archive them
  • Performs experiments that identify optimized conditions for preservation of bacterial strains of interest.
  • The incumbent will be responsible for performing experiments that create new cell banking and testing strategies for both laboratory strains and those of commercial interest
  • Performs laboratory experiments in fermentation and cell culture that evaluate strains, media and expression strategies for production of vaccine antigen candidates
  • Incumbent assists senior researchers in the evaluation of new technologies for the group
  • Incumbent evaluates and applies new analytical methods for the group
  • Gains understanding of the discipline through reading of the scientific literature
  • Organizes and tabulates data
  • Presents data summaries within the group
  • Fully documents experimental results in notebooks in a timely and compliant manner.
  • Works irregular hours (weekends and outside of 9-5 format) as experiments warrant
  • QUALIFICATIONS

  • MS degree in microbiology, biotechnology, bioengineering or microbial genetics with 3-5 years of relevant experience required
  • Has a solid understanding of microbiology and molecular biology and/or microbial genetics
  • Has an appreciation of fermentation and cell culture techniques
  • Has previously worked in a mirobiology, biotechnology or microbial genetics laboratory setting
  • Experience in formal microbial cell banking and familiarity with testing protocols and guidelines is preferred
  • Experience in the genetic manipulation of E. coli and/or other industrially important gene expression hosts is preferred
  • Has knowledge of cGMP and regulatory requirements as they apply to preparation of cGMP cell banks
  • Has experience with analytical techniques such as SDS-PAGE and HPLC
  • Ability to organize and execute experiments
  • Ability to work with limited supervision
  • Collaborates in a team environment
  • Has a proven ability to organize and tabulate data
  • Sunshine Act

    Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider s name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

    EEO & Employment Eligibility

    Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

    N (Other) (United States of America)

    Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.


    Associated topics: bacteria, bio, bioengineering, biology, immunobioengineering, life science, neurobiology, pathophysiology, physiology, protein * The salary listed in the header is an estimate based on salary data for similar jobs in the same area. Salary or compensation data found in the job description is accurate.

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